Toxicity Study of Gelatin-chitosan Films with Bone Marrow Stromalcells Cultured in Rat

نویسندگان

  • Afsane Karami Juyani Researcher, Aja University of Medical Siences, Tehran, Iran.
  • Gholamreza Kaka Assistant Professor, Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • Mahvash Jafari Assistant Professor, Department of Biochemistry, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • Maliheh Taghizadeh PhD Candidate of Medical University, Tabiat Modares University. Instructor, Aja University of Medical Siences, Tehran, Iran.
  • Mehdi Saberi Proffesor, Department of Pharmacology, Baqiyatallah University of Medical Sciences
  • Simin Riyahi PhD of Exercise Physiology, Instructor, Aja University of Medical Siences, Tehran, Iran.
چکیده مقاله:

Background Gelatin and chitosan are known as biodegradable and biocompatible biopolymers.These biopolymers have recently receivedincreasingly more attention for tissueengineering.The aim of this study was to evaluate the toxicity effects of gelatin-chitosan film on bone marrow stromal cell(BMSCs) culture in rat.                                                  Material & Methods: First, gelatin- chitosan composites film were prepared by solution mixing, followed by film casting of both biopolymers in glacial acetic acid.After two passage of BMSCs culture, the cells were cultured in four plate groups including: control plates have no any film,gelatinplates,chitosan plates and gelatin- chitosan plates.Theproliferation,differentiation,viability and apoptosis rates of BMSCs were studied duringthesecond,fourthand sixth days.The activity of superoxidedismutase(SOD),catalase(CAT)and  glutathione(GSH) and malondialdehyde(MDA) levels were determined by using biochemical methods. Results: The results showed that the mean BMSCs proliferation significantly reduced in chitosan group compared to control group (p

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toxicity study of gelatin-chitosan films with bone marrow stromalcells cultured in rat

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عنوان ژورنال

دوره 2  شماره 2.3

صفحات  87- 87

تاریخ انتشار 2014-05-01

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